Facilities: Metabolomics Core The Laboratory for Metabolics and Macromolecular Analysis at the Redox Biology Center in cooperation with the mass spectrometry facility at the chemistry department supports scientists within the RBC and the Univerity of Nebraska. This laboratory provides mass spectrometry services for proteomic analysis including protein identification, mapping protein-protein interactions, and characterization of protein modifications such as phosphorylation and glycosylation, and for analysis of metabolites, either in context of metabolomics or metabolite profiling. We are very interested in the collaborations with scientists at UNL and UNMC in studying systems of biomedical importance. The instrumentation is located in E156 of the Beadle Center. Currently we have an API QSATR XL mass spectrometer and an LC Packings Capillary HPLC system. The QSATR is a tandem mass spectrometer (MS/MS) with the QqTOF configuration and offers the option to interface with nano/capillary-HPLC, nanospray, and MALDI plate. The accuracy and reproducability of the time-of-flight analyzer enable accurate mass measurements to be carried out with small organic molecules and the excellent resolution makes charge state indentification routine. In the MS/MS mode, the two analyzers are used together for structural or sequence studies by monitoring fragmentation patterns in molecules. The LC Packings Capillary HPLC system with SWITCHOS enables two-dimensional (strong cation exchange and reversed phase) chromatographic separation. This integrated LC-MS/MS system in combination with ICATTM offers accurate, rapid and sensitive identification and quantitation of complex protein mixtures. Contact: Dr. Ashraf Raza (402) 472-3828 located at E154 Beadle Center. Services Offered by RBC Mass Spectrometry Core FacilityRBC MS Facility is equipped with 4000 Qtrap, ABI (Quadrupole Ion Trap) and Q-Star XL ABI (Quadrapole-TOF) tandem mass spectrometers to provides a wide range mass analysis. These instruments are equipped with ESI (microspray and nanospray) and APCI sources to provide a wide range of ionization capabilities. Nano flow LC-Packings UltiMate and Micro flow Shimatzu high pressure liquid chromatography (HPLC) systems are also available for LC-MS or LC-MS/MS analysis of complex analytes mixtures. Gel-based or in-solution proteins are digested and analyzed using LC MS/MS. Proteins are identified using in-house Mascot Data Base search. MS-based methods are also available for the identification and mapping of post-translational modification of proteins. Following type of analysis may be performed in this facility.
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