Dr. Angela K. Pannier, Associate Professor
Biological Systems Engineering
University of Nebraska-Lincoln
231 Chase Hall
Lincoln, NE 68583-0726
Gene Delivery Tool (UNL Office of Research Video)
The long-term goal of the Pannier Lab is to understand and design innovative biomaterials and gene delivery systems to advance biotechnology, diagnostics, fundamental understanding of embryology and tissue development, and regenerative medicine therapies. Research projects within the Pannier Lab are focused in three different themes including nonviral gene delivery, tissue engineering, and protein-cell-biomaterial interactions.
Within the nonviral gene delivery theme, our aim is to determine and understand the mechanisms that render cells responsive to the transfer of genetic material (e.g. DNA), concentrating on the cell microenvironment, the interaction between cells and biomaterials, and the intracellular processes and subsequent signaling involved during nonviral gene delivery. Within the tissue engineering theme, our objective is to develop biomaterial scaffolds and culture systems to understand and promote tissue, organ, and organism development, regeneration, and growth. Within the protein-cell-material interaction theme, projects aim to make use of a novel combinatorial spectroscopic ellipsometry and quartz crystal microbalance with dissipation analytical technique to uncover new and unique information on processes that occur at biomaterial interfaces, which are critical to the performance of biomaterials in biotechnological and therapeutic applications.
The experimental approaches developed in the Pannier Lab provide valuable insights to the fields of gene delivery and biomaterials, with very promising potential for future scientific discovery and translation to therapeutic, biotechnological, and tissue engineering applications.
PublicationsRodenhausen KB, Schmidt D, Kasputis T2, Pannier AK, Schubert E, and Schubert M. 2012. Generalized ellipsometry in-situ quantification of organic adsorbate attachment within slanted columnar thin films. Optics Express. 20(5):5419-28. (20% contribution)
Othman SF, Curtis ET, Plautz SA, Pannier AK, Butler SD, Xu H. 2012. Magnetic Resonance Elastography monitoring of tissue engineered constructs. NMR in Biomedicine. 25(3):452-63.
Sharp AT, Pannier AK, Wysocki BJ, Wysocki TA. 2012. A novel telecommunications-based approach to HIV modeling and simulation. Nano Communication Networks. 3(2): 129-137.
Plautz SA, Boanca G, Riethoven J-JM, Pannier AK. 2011. Microarray Analysis of Gene Expression Profiles in Cells Transfected with Nonviral Vectors. Molecular Therapy 19(12):2144-51.
Rodenhausen KB, Kasputis T2, Pannier AK, Gerasimov JY, Lai RY, Solinsky M, Tiwald TE, Wang H, Sarkar A, Hofmann T, Ianno N, and Schubert M. 2011. Combined optical and acoustical method for determination of thickness and porosity of transparent organic layers below the ultra-thin lm limit. Review of Scientific Instruments. Oct; 82(10):103111.
Rodenhausen KB, Duensing BA1, Kasputis T2, Pannier AK, Hofmann T, Schubert M, Tiwald TE, Solinsky M, and Wagner M. 2011. In-situ monitoring alkanethiol self-assembled monolayer chemisorption with combined spectroscopic ellipsometry and quartz crystal microbalance techniques. Thin Solid Films. 519(9): 2817-2820.
Singh D1, Pannier AK, Zempleni J. 2011. Identification of holocarboxylase synthetase chromatin binding sites using the DamID technology. Anal Biochem. 413(1):55-9.
Bellis AD, Bernabé BP, Weiss MS, Yarrington ME, Barbolina MV, Pannier AK, Jeruss JS, Broadbelt LJ, Shea LD. 2011. Cellular arrays for large-scale analysis of transcription factor activity. Biotechnol Bioeng. 108(2):395-403.
Pannier AK, Wieland JA, Shea LD. 2008. Incorporation of polyethylene glycol into self-assembled monolayers enhances substrate-mediated gene delivery by nonspecifically- bound complexes. Acta Biomat. 4:26-39.
Pannier AK, Ariazi EA, Bellis AD, Bengali Z, Jordan VC, Shea LD. 2007. Bioluminescence imaging for assessment and normalization in transfected cell arrays. Biotechnol Bioeng. 98:486-97.
Pannier AK, Anderson BC, Shea LD. 2005. Substrate-mediated delivery from self-assembled monolayers: Effect of surface ionization, hydrophilicity, and patterning. Acta Biomat. 1:511-522.
Bengali Z, Pannier AK, Segura T, Anderson BC, Jang JH, Mustoe TA, Shea LD. 2005. Gene delivery through cell culture substrate adsorbed DNA complexes. Biotechnol Bioeng. May 5; 90(3):290-302.
Pannier AK, Shea LD. 2004. Controlled release systems for DNA delivery. Mol. Ther. 10: 19-26.
Pannier AK, Arora V, Iversen PL, Brand. 2004. Transdermal delivery of phosphorodiamidate Morpholino oligomers across hairless mouse skin. Int. J. Pharm. 275:217-226.
Pannier AK, Brand, RM, Jones DD. 2003. Fuzzy modeling of skin permeability coefficients. Pharm. Res.20: 143-148.
2.1.1 Peer Reviewed Journal Publications Accepted for Publication/In Press
Wysocki BJ, Martin TM2, Wysocki TA, and Pannier AK. 2013. Modeling nonviral gene delivery as a macro-to-nano communication system. Nano Communication Networks (in press).
Books, Chapters and Reviews
- Pannier AK and Segura T. 2013. Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles. In: Ogris M and Oupicky D (eds) Nanotechnology for Nucleic Acid Delivery: Methods in Molecular Biology. 948:149-69. (50% contribution)
- Segura, T., Shea, L.D., Pannier, A.K., Bengali. Z., Jang, J.H., Chung, P., and Anderson, B. Controlled surface-associated delivery of genes and oligonucleotides. U.S. Patent No. 7,029,697. April 18, 2006.
- Pannier, A.K., Ariazi, E.A., Jordan, V.C., and Shea, L.D. Cellular arrays. U.S. Patent Application: 11/809,772. Filing Date: June 1, 2007.