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Current Research
A major goal of the Pannier Lab is to understand the mechanisms which render cells responsive to DNA transfer, concentrating on the extracellular environment of the cell, the interaction between cells and biomaterials, as well as the intracellular processes and subsequent signaling involved during nonviral gene delivery. With a better understanding of gene transfer, we expect to use that knowledge to design more efficient delivery systems, which could be used for gene therapy or tissue engineering applications. The Pannier Lab is also interested in developing new polymeric methods of nonviral gene delivery that facilitate the application of gene transfer to oral delivery. In addition, we are applying gene delivery methods to develop techniques to measure epigenetic profiles in small cell population. Furthermore, we are interested in understanding environmental effects of culture of tissue engineering constructs on epigenetic profiles that may dictate the efficacy of engineered tissue. Finally, we are developing novel techniques to pattern hydrogels for tissue engineering scaffolds, using fiber-templating to create conduits within the matrix for directed cell growth, as well as applying tissue engineering approaches to study embryology. The experimental approaches developed in our lab provide valuable insights to the gene delivery and biomaterial fields, and our goal is to translate our discoveries to therapeutic, biotechnological, and tissue engineering applications.
Recent Publications
Pannier AK, Brand, RM, Jones DD. 2003. Fuzzy modeling of skin permeability coefficients. Pharm. Res. 20: 143-148.
Pannier AK, Arora V, Iversen PL, Brand. 2004. Transdermal delivery of phosphorodiamidate Morpholino oligomers across hairless mouse skin. Int. J. Pharm. 275:217-226.
Pannier AK, Shea LD. 2004. Controlled release systems for DNA delivery. Mol. Ther. 10: 19-26.
Bengali, Z., Pannier AK, Segura T, Anderson BC, Jang JH, Mustoe TA, Shea LD. 2005. Gene delivery through cell culture substrate adsorbed DNA complexes. Biotechnol Bioeng. May 5; 90(3):290-302.
Pannier AK, Anderson BC, Shea LD. 2005. Substrate-mediated delivery from self-assembled monolayers: Effect of surface ionization, hydrophilicity, and patterning. Acta Biomat. 1:511-522.
Pannier. A,K., Ariazi. E.A., Bellis, A.D., Bengali, Z., Jordan, V.C., Shea, L.D. 2007. Bioluminescence imaging for assessment and normalization in transfected cell arrays. Biotechnol Bioeng. 98:486-97.
Pannier, A.K., Wieland, J.A., Shea, L.D. 2008. Incorporation of polyethylene glycol into self-assembled monolayers enhances substrate-mediated gene delivery by nonspecifically- bound complexes. Acta Biomat.:26-39.
Patents
Segura, T., Shea, L.D., Pannier, A.K., Bengali. Z., Jang, J.H., Chung, P., and Anderson, B. Controlled surface-associated delivery of genes and oligonucleotides. U.S. Patent No. 7,029,697. April 18, 2006.
Pannier, A.K., Ariazi, E.A., Jordan, V.C., and Shea, L.D. Cellular arrays. U.S. Patent Application: 11/809,772. Filing Date: June 1, 2007.
