My current research interests are in the area of biomaterial development and further use of these biomaterials in (a) bioseparations, and (b) biomedical applications. My research in biomaterials and tissue engineering focuses on the synthesis and development of novel biofunctional materials for potential biomedical applications synthesized from natural polymers and ceramics. I also have a research focus on the engineering of mammalian cell lines to produce therapeutic proteins. Brief descriptions of current projects are provided below.
Bioseparation Research Area: Separation of biological molecules is a persistent and costly problem in downstream operations. Most biological separation operations involve chromatography and it is an improtant tool in the pharmaceutical industry.
My research interests revolve around support systems used in chromatographic separations. In particular I am interested in a highly thermally and mechanically stable ceramic called zirconia. With regard to zirconia, I am primarily interested in the synthesis and characterization of porous zirconia particles.
The other support systems that pique my interest are the so called pseudo-affinity matrices. These systems have been around for a while and are currently being extensively used in some form or the other, however little is known bout the true mechanisms with which these systems are able to carry out their functions.
Biomaterial Research Area: The other area of interest to me is biomaterials. Biomaterials may be crudely defined as any natural or synthetic material that performs a desired funtion by interfacing with biological fluids or living tissue with little or no side effect. All prosthesis materials are biomaterials, but so are the polymers we use in Band Aid®.
Part of my research deals with the preparation of blended scaffolds using natural and synthetic polymers for seeding cells.
Also of interest to me is the use of these biomaterials in the synthesis of nano-particles. I am collaborating to make nano-shells that can be used for drug delivery purposes.
Subramanian, A., Lin, H., Vu, D and Larsen, G. F., “Synthesis and Evaluation of Scaffolds for Potential Use in Cartilage Tissue Engineering”, In Publication, Proceedings of the Rocky Mountain Bioengineering Symposium 2004 (ISA society).
Subramanian, A., Sarkar, S., Woollam, J. A. and Nosal, W., “Synthesis and Characterization of Albumin Binding Surfaces fro Implantable Devices”, In Publication, Proceedings of the Rocky Mountain Bioengineering Symposium 2004 (ISA society).
Kunjachan, V., Subramanian, A., Hanna, M and Guan, J. J. “Comparison of Different Fabrication Techniques Used for Processing 3-D, Porous, Biodegradable Scaffolds from Modified Starch for Bone Tissue Engineering”, In Publication, Proceedings of the Rocky Mountain Bioengineering Symposium 2004 (ISA society).
Sarkar, S., McNeff, C. V., Carr. P. W., and Subramanian, A., “Characterization and Optimization of a Chromatographic Process Based on Zirconia Particles”, Journal of Chromatography 790, 143-152 (2003).
Subramanian, A and Sarkar, S., “Interaction of hIgG with Modified EDTPA-modified Zirconia”, Journal of Chromatography A 989, 131-138 (2003).
Subramanian, A. and Sarkar, S., “The Use of EDTPA-Modified Zirconia in the Separation of Immunoproteins”, Journal of Chromatography 944(1-2), 179-187 (2002).
Subramanian, A, Carr, P.W., and McNeff, C. V. “Use of Spray Dried Zirconia Microspheres in the Separation of Immunoglobulins from Cell Culture Supernatant”, Journal of Chromatography 890, 15-23 (2000).
Subramanian, A. and Martinez, B. “The Use of DVS Activated Cellulose Beads in Large-scale Bioprocess Chromatography: Purification of Human Antibodies”, International Journal of Bio-Chromatography 5(1), 31-47 (2000).