Gilles J. Basset

Assistant Professor - Department of Agronomy and Horticulture / Department of Biochemistry

(402) 472-2930 | Email

Research Interests

Research in our laboratory aims at understanding how plants synthesize and metabolize chemicals that are beneficial to human health, and at applying this knowledge to improve the nutritional value of plant-based foods. Our approach is integrative and combines genomics, genetics, enzymology, metabolic biochemistry, organic chemistry, cellular biology and metabolic engineering. We are especially interested in metabolic branches that involve multifunctional enzymes, metabolic channeling of biosynthetic intermediates, and enzymes that have evolved novel functions in plants. Our current research focuses on plant naphthoquinones.

Gilles J. Basset is the recipient of the 2011-2012 Junior Faculty Recognition for Excellence in Research; Branham Endowment Fund; Agricultural Research Division, Institute of Agriculture and Natural Resources, University of Nebraska-Lincoln.

Research in the Basset laboratory is made possible by National Science Foundation grant MCB-0918258 (08/2009-07/2012); 'Phylloquinone biosynthesis in plants: enzyme discovery and pathway flux control'.

 

Recent Papers

Recent Publications

Ducluzeau AL, Wamboldt Y, Elowsky CG, Mackenzie SA, Schuurink RC, Basset GJ (2011) Gene network reconstruction identifies the authentic trans-prenyl diphosphate synthase that makes the solanesyl moiety of ubiquinone-9 in Arabidopsis. The Plant Journal (in press) doi: 10.1111/j.1365-313X.2011.04796.x.

Fitzpatrick TB, Basset GJ, Borel P,  Carrari F, DellaPenna D, Fraser PD, Hellman H, Osorio-Algar S, Rothan C, Valpuesta V, Caris-Veyrat C, Fernie AR (2011) Vitamin deficiency: Can plant science help? The Plant Cell (in press).

Van Oostende C, Widhalm JR, Furt F, Ducluzeau AL, Basset GJ (2011) Vitamin K1 (phylloquinone): function, enzymes and genes. Advances in Botanical Research 59, 229-261.

Xu YZ, Arrieta-Montiel MP, Wamboldt YJ, Virdi K,  de Paula WBM, Widhalm JR, Basset GJ, Davila JI, Elthon TE, Elowsky CG, Sato SJ, Clemente TE, Mackenzie SA. (2011) MSH1 is a multi-functional protein in plants that alters mitochondrial and plastid properties and response to high light. The Plant Cell 23, 3428-3441.

Boulila-Zoghlami L, Gallusci P, Holzer FM, Basset GJ, Djebali W, Chaibi W, Walling LL, Brouquisse R. (2011) Up-regulation of leucine aminopeptidase-A in cadmium-treated tomato roots. Planta 234,857-863.

Furt F., van Oostende C., Widhalm J.R., Dale, M.A., Wertz, J., Basset G.J. (2010) A bimodular oxidoreductase mediates the specific reduction of phylloquinone (vitamin K1) in chloroplasts. The Plant Journal 64, 38-46.

Widhalm J.R., van Oostende, C., Furt F., Basset, G.J. (2009) A dedicated thioesterase of the Hotdog-fold family is required for the biosynthesis of the naphthoquinone ring of vitamin K1. Proceedings of the National Academy of Sciences of the USA 106, 5599-5603.

van Oostende, C, Widhalm J.R., Basset, G.J. (2008) Detection and quantification of vitamin K1 quinol in leaf tissues. Phytochemistry 69, 2457-2462.

Kim, H.U., van Oostende, C., Basset, G.J., Browse, J. (2008) The AAE14 gene encodes the Arabidopsis o-succinylbenzoyl-CoA ligase that is essential for phylloquinone synthesis and photosystem I function. The Plant Journal 54, 272-283.

Bozzo, G.G., Basset, G.J., Naponelli, V., Noiriel, A., Gregory, J.F. 3rd, Hanson, A.D. (2008) Characterization of the folate salvage enzyme p-aminobenzoylglutamate hydrolase in plants. Phytochemistry 69, 29-37.

Orsomando, G., Bozzo, G.G., Díaz de la Garza, R., Basset, G.J.C., Quinlivan E. P., Naponelli, V., Rébeillé, F., Ravanel, S., Gregory III, J. F., Hanson A. D. (2006) Evidence for folate salvage reactions in plants. The Plant Journal 46, 426-435.

Sahr, T., Ravanel, S., Basset, G., Nichols, B.P., Hanson, A. D., Rébeillé, F. (2006) Folate synthesis in plants: purification, kinetic properties, and inhibition of aminodeoxychorismate synthase. The Biochemical Journal 396, 157-162.

Klaus, M.J.S., Kunji, E.R.S., Bozzo, G.G., Noiriel, A., Díaz de la Garza, R., Basset, G.J.C., Ravanel, S., Rébeillé, F., Gregory III, J.F., Hanson, A.D. (2005). Higher plant plastids and cyanobacteria have folate carriers related to those of trypanosomatids. Journal of Biological Chemistry 280, 38457-38463.

Basset, G.J.C., Quinlivan, E.P., Gregory III, J.F., Hanson, A.D. (2005). Folate synthesis and metabolism in plants, and prospects for biofortification. (Review). Crop Science 45, 449-453.

Basset, G.J.C., Ravanel, S., Quinlivan, E.P., White, R., Giovannoni, J.J., Rébeillé, F., Nichols, B.P., Shinozaki, K., Seki, M., Gregory III, J.F., Hanson, A.D. (2004). Folate Synthesis in Plants: The Last Step of the p-Aminobenzoate Branch is Catalyzed by a Plastidial Aminodeoxychorismate Lyase. The Plant Journal 40, 453-461.

Díaz de la Garza, R., Quinlivan, E.P., Klaus, M.J.S., Basset, G.J.C., Gregory III, J.F., Hanson, A.D. (2004). Folate Biofortification in Tomatoes by Engineering the Pteridine Branch of Folates Synthesis. Proceedings of the National Academy of Sciences of the USA 101, 13720-13725.

Basset, G.J.C., Quinlivan, E.P., Ravanel, S., Rébeillé, F., Nichols, B.P., Shinozaki, K., Seki, M., Adams-Philips, L.C., Giovannoni, J.J., Gregory III, J.F., Hanson, A.D. (2004). Folate synthesis in plants: The p-aminobenzoate branch is initiated by a bifunctional PabA-PabB protein that is targeted to plastids. Proceedings of the National Academy of Sciences of the USA 101, 1496-1501.

Quinlivan, E.P., Roje, S., Basset, G., Shachar-Hill, Y., Gregory III, J.F., Hanson, A.D. (2003) The folate precursor p-aminobenzoate is reversibly converted to its glucose ester in the plant cytosol. Journal of Biological Chemistry 278, 20731-20737.

Basset, G., Quinlivan, E.P., Ziemak, M. J., Díaz de la Garza, R., Fischer, M., Schiffmann, S., Bacher, A., Gregory III, J.F., Hanson, A.D. (2002) Folate synthesis in plants: The first step of the pterin branch is mediated by a unique bimodular GTP cyclohydrolase I. Proceedings of the National Academy of Sciences of the USA 99, 12489-12494.

Rontein, D., Basset, G*., Hanson, A, D. (2002) Metabolic engineering of osmoprotectant accumulation in plants. (Review). Metabolic Engineering. 4, 49-56.

Basset, G., Malek, L., Brouquisse, R., Raymond, P. (2002) Changes in the expression and the enzymic properties of the 20S proteasome in sugar-starved maize roots. Evidences for an in vivo oxidation of the proteasome. Plant Physiology. 128, 1149-1162.