The Nebraska Center for Virology Core Facilities
Flow Cytometry Core Facility
Nebraska Center for Virology
Director: Dr. Charles Kuszynski
The NCV Flow Cytometry Core Facility provides flow cytometry services
to members of the Nebraska Center for Virology and investigators
on and off the UNL campus on a fee for service basis. The core provides
comprehensive data collection, data interpretation and education
in flow cytometry.
The NCV locations are:
E310 Beadle Center; phone: 472-4559
116A VBS; phone 472-3142
Please contact Daneille Shea to schedule
services. The facility director, Dr. Charles Kuszynski is available
for experimental design assistance. [ckuszyns@unmc.edu]
SERVICES AVAILABLE
ANALYSIS
Immunophenotyping (1-4 colors)
Gene Transduction/Expression
Green, Yellow & Red Fluorescent Proteins
Receptor Expression
DNA Ploidy Analysis
Cell Cycle Analysis
Apoptosis (A0)
Annexin V binding
Caspase Activity
Kinetic Studies
New Protocol Development
CELL SORTING
Cell Sorting
Cell Enrichment and Purification
Single Cell Cloning
INSTRUMENTATION
The UNL facilities house two bench top analyzers and one cell sorting
instrument.
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B-D FACSCalibur dual-laser cytometer capable of four color,
8 parameter analysis operating under CellQuest,
-
B-D FACScan
single-laser cytomer capable of 3 color, 6 parameter analysis
operating under CellQuest
-
B-D FACSVantage SE three-laser, high speed
cell sorter capable of 5 color, 8 parameter analysis, operating
under CellQuest
Also available to all faculty are the following instruments and
services at the UNMC Cell Analysis Facility in Omaha
-
(2) B-D FACSCalibur dual-laser cytometers capable of four
colors, 8 parameter analysis operating under CellQuest
-
B-D LSR II three-laser cytometer capable of 10 colors,
12 parameter analysis operating under FACSDiva
-
B-D FACSVantage
DivA three-laser, high speed cell sorter capable of 6 colors,
10 parameter analysis, operating under FACSDiva and CellQuest Pro
-
Becton
Dickinson FACStarPlus three-laser low pressure cell sorter
capable of 4 colors, 6 parameter analysis, operating under CellQuest
-
Miltenyi
AutoMACs automated magnetic cell separation system.
UNMC Services can be arranged by calling the laboratory manager
Linda Wilkie
Cell analysis Facility
UNMC
402 559-6267
lwilkie@unmc.edu
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