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University of Nebraska–Lincoln

Nebraska Center for Virology

The Strength of Cooperation Among Three Institutions

The Nebraska Center for Virology Core Facilities

Flow Cytometry Core Facility
Nebraska Center for Virology
Director: Dr. Charles Kuszynski

The NCV Flow Cytometry Core Facility provides flow cytometry services to members of the Nebraska Center for Virology and investigators on and off the UNL campus on a fee for service basis. The core provides comprehensive data collection, data interpretation and education in flow cytometry.

The NCV locations are:
E310 Beadle Center; phone: 472-4559
116A VBS; phone 472-3142

Please contact Daneille Shea to schedule services. The facility director, Dr. Charles Kuszynski is available for experimental design assistance. [ckuszyns@unmc.edu]

SERVICES AVAILABLE

ANALYSIS
Immunophenotyping (1-4 colors)
Gene Transduction/Expression
Green, Yellow & Red Fluorescent Proteins
Receptor Expression
DNA Ploidy Analysis
Cell Cycle Analysis
Apoptosis (A0)
Annexin V binding
Caspase Activity
Kinetic Studies
New Protocol Development

CELL SORTING
Cell Sorting
Cell Enrichment and Purification
Single Cell Cloning

INSTRUMENTATION

The UNL facilities house two bench top analyzers and one cell sorting instrument.

  • B-D FACSCalibur dual-laser cytometer capable of four color, 8 parameter analysis operating under CellQuest,

  • B-D FACScan single-laser cytomer capable of 3 color, 6 parameter analysis operating under CellQuest

  • B-D FACSVantage SE three-laser, high speed cell sorter capable of 5 color, 8 parameter analysis, operating under CellQuest

Also available to all faculty are the following instruments and services at the UNMC Cell Analysis Facility in Omaha

  • (2) B-D FACSCalibur dual-laser cytometers capable of four colors, 8 parameter analysis operating under CellQuest

  • B-D LSR II three-laser cytometer capable of 10 colors, 12 parameter analysis operating under FACSDiva

  • B-D FACSVantage DivA three-laser, high speed cell sorter capable of 6 colors, 10 parameter analysis, operating under FACSDiva and CellQuest Pro

  • Becton Dickinson FACStarPlus three-laser low pressure cell sorter capable of 4 colors, 6 parameter analysis, operating under CellQuest

  • Miltenyi AutoMACs automated magnetic cell separation system.

UNMC Services can be arranged by calling the laboratory manager
Linda Wilkie
Cell analysis Facility
UNMC
402 559-6267
lwilkie@unmc.edu