Core Facilities
   - Flow Cytometry
   - Microscopy
   - Structural Protein

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   - Fogarty AITRP
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   - Undergraduate Summer
     Research Experience in
     Virology (USREV)

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	U.S. Department of Health and Human Services - National Institutes of Health
	National Center for Research Resources

The Nebraska Center for Virology Core Facilities

Structural/Protein Core
UNL Department of Chemistry
Director: Dr. Ronald Cerny

This core supports all aspects of protein work, from purification to structural analysis. The structural biochemists from this core are contributing innovative molecular approaches for studying viral-host interactions, broadening the Center's research tools to carry inter-disciplinary research.

Activity/Service/Equipment Available:

• Exact Mass Measurement – Electron Ionization MS
• Low Resolution Fast Atom Bombardment (FAB) MS
• Exact Mass Measurement – FAB MS
• Direct Injection (single component) Electrospray Ionization (ESI)
• Liquid Chromatography – Mass Spectrometry (LC/MS)
• Matrix Assisted Laser Desorption Ionization MS (MALDI) Peptide
• Matrix Assisted Laser Desorption Ionization MS (MALDI) Protein
• 2D gel Image Analysis
• Robotic Gel Excision
• In-gel enzymatic digestion (1D and 2D Gels)
• Protein Identification (LC/MS-MS and database searching)
• Mapping Post-Translational Modifications
• Assistance in the development of LC/MS methods for biomolecules