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   - Structural Protein

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	U.S. Department of Health and Human Services - National Institutes of Health
	National Center for Research Resources

Lawrence Parkhurst, PhD

Professor
University of Nebraska-Lincoln
Department of Chemistry
525 Hamilton Hall
Lincoln, NE 68588-0304
Phone - 402.472.3503
Fax - 402.472.2044
lparkhurst1@unl.edu

Select Publications: (since 1996)

Parkhurst, K.M. and Parkhurst, L.J. (1996) "Detection of Point Mutations in DNA by Fluorescence Energy Transfer@, J. of Biomedical Optics, 1: 435-441.
Parkhurst, K.M., Brenowitz, M. and Parkhurst, L.J. (1996) "Simultaneous Binding and Bending of Promoter DNA by TBP: Real Time Kinetic Measurements" Biochemistry 35: 7459-7465.
Parkhurst, K.M., Richards, R.M., Brenowitz, M. and Parkhurst, L.J. (1999) "Intermediate species possessing bent DNA are present along the Pathway to Formation of a Final TBP-TATA Complex" J. Mol. Biol. 289: 1327-1341.
Parkhurst, L.J., Parkhurst, K.M., Wu, J. and Richards, R. "Structural & Functional Effects of osmolytes on TBP-TATA Box Interactions", Abstract # 281, 44th Ann. Mts. Biophysical Soc., New Orleans, LA. Feb. 12-16, 2000.
L. Parkhurst. "Some Aspects of Fluorescence of Particular Relevance for Biological Processes", (in press) Book Chapter, "Near Infrared Applications in Biotechnology, Ramesh Raghavachari, ed. Marcel-Dekker, New York.
Wu, J., Parkhurst, K., Powell, R., Brenowitz, M. and Parkhurst, L. (2000) "Variable DNA Bending in Solution in TBP-DNA Complexes (submitted).

Education:

Yale University, New Haven, CT
BS, Chemistry, 1959

Yale University, New Haven, CT
MS, Chemistry, 1960

Yale University, New Haven, CT
PhD, P. Chemistry/Mol. Spec., 1960

U.PA, Johnson Foundation, Philadelphia, PS
Postdoctoral, Biophysics, 1965-66

Cornell University, Ithaca, NY
Postdoctoral, Biophysics, Biochemistry, 1966-68

Professional Positions:

1992
Postdoctoral Associate
University of Wisconsin,
Madison, WI

1976-
Prof. of Chemistry
University of Nebraska, Lincoln, NE

1973-
Joint Appointment
School of Biological Sciences
University of Nebraska, Lincoln, NE

1985-1989
Member, NIH Molecular and Cellular Biophysics Study Section

1987
Visiting Prof. Molecular Biology and Genetics,
Univ. of Naples

1973-1976
Assoc. Professor, Chemistry
University of Nebraska, Lincoln, NE

1969-73
Summer, Assistant Professor of Chemistry
University of Nebraska, Lincoln, NE

1970
Visiting Prof. of Biochemistry
C.N.R., Naples, Italy,

1967-68
NIH Special Fellow, Section of Mol Biol & Biochem
Cornell Univ, Ithaca, NY

1967
Investigator,
Marine Biological Lab
Woods Hole, MA,

Honors & Awards:

Victor Wilson Scholarship, Yale U., 1955-1959

Phi Beta Kappa (Junior Year at Yale)
Summa Cum Laude, 1959

Highest Class Dept Honors, Chemistry, 1959

Sigma Xi, 1959

NSF Predoctoral Fellow, 1959-1962

Fellow of Yale U., 1963-1965

NIH Postdoctoral, 1965-1967

NIH Special Fellow, 1968-1969

Visiting Prof. Biochemistry, C.N.R., Lab. of Molecular Embryology, Naples, Italy, Summer 1970

Visiting Scholar, China, 1980

Burroughs-Wellcome Fellowship, Cambridge U., Summer 1980

Fellowship for Neurobiophysics, Woods Hole Marine Biol. Lab., May 1 1984

Visiting Prof., Univ. of Naples, Biophysics, Naples, Italy, 1987

Member of BBCA Study Section, 1985-1989