Flow Cytometry Core
116A VBS and E310 Beadle
Services are Available to the University Community and are Free
of Charge through September 30, 2005
The Flow Cytometry Core is now fully functional and provides comprehensive
data collection, data analysis, data interpretation and education
in flow cytometry to researchers of the University of Nebraska.
What is Flow Cytometry
Flow Cytometry, typically using fluorescent probes which bind to
specific cell associated molecules, allows measurements of various
phenotypic, biochemical and molecular characteristics of individual
cells (or particles) suspended in a fluid stream. As the cells
flow past a focused laser beam, the probes fluoresce and the emitted
light is collected and directed to appropriate detectors. These
detectors, in turn, translate the light signals into electronic
signals proportional to the amount of light collected. Signals
are then converted to graphics for visual and digital analysis.
Services Available
The ability of flow cytometers to evaluate cells at an extremely
rapid rate (e.g. up to 20,000 events per second) makes this technology
ideally suited for the reliable and accurate quantitative analysis
of selected physical properties of cells of interest. The sensitivity
of these instruments for detecting the presence of molecules expressed
at low levels is impressive; given high quality cell preparations
and reagents, as few as 200 molecules per cell may be detected.
| Immunophenotyping (1-4 colors) |
Cell Sorting |
| Gene Transduction/Expression |
Cell Enrichment and Purification |
| Green, Yellow & Red Fluorescent Proteins |
Single Cell Cloning |
| Receptor Expression |
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| DNA Ploidy Analysis |
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| Cell Cycle Analysis |
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| Apoptosis (A0) |
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| Annexin V Binding |
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| Caspase Activity |
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| Kinetic Studies |
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| New Protocol Development |
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Who to Contact
Please contact Danielle Shea, (402) 472-4559, to schedule services
one day in advance. The facility director, Dr. Charles Kuszynski,
(402) 559-6299, is available by appointment for experimental design
assistance and data interpretation.
A Shared Resource of UNL
Nebraska Center for Virology and the Center for Biotechnology
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