USREV: Virology

For information contact

Tori Solomon
4240 Fair Street
102 MOLR
402-472-4560
tori.solomon@unl.edu 

2017 Virology USREV students.
2017 Virology USREV students.

Application Dates

Nov 15 2017 App opens
February 1 Priority deadline
March 1 App closes
April 1 Decisions complete

Program Dates

June 3 2018 Arrival day
June 4 Program begins
August 7 Program ends
August 8 Departure day

Who should apply


Related fields

  • Biology
  • Biochemistry
  • Microbiology
  • Molecular Biology

This program gives preference to and encourages applications from students whose primary interest is exploring a career in biomedical research.

Eligibility

Participation in the Nebraska Summer Research Program is limited to students who meet the following criteria:
  • U.S. Citizen or Permanent Resident
  • Current undergraduate with at least one semester of coursework remaining before obtaining a bachelor's degree

See Eligibility for more information.

How to apply

Follow the application steps to submit the following materials.

About the Program

The Nebraska Center for Virology (NCV) faculty research challenging topics addressing problems of epidemic proportions facing mankind including AIDS and HIV replication and pathogenesis, herpesvirus latency and cancer, human papilloma virus and cancers, and chlorellavirus biology.  Our goals include designing novel vaccines and therapeutic strategies to block disease.

Each year, NCV invites applications for our Undergraduate Summer Research Experience in Virology (USREV) program from students enrolled at institutions in Nebraska as well as other states, who are motivated to explore the exciting opportunities for careers as scientists in biomedical research, while working with our experienced research faculty along side current graduate students and postdoctoral research associates in their labs. 

As a selected student, you will design and implement a tailor-made research project.  You will then conduct exciting and challenging experiments to test hypotheses under the guidance and mentoring of our expert faculty.  As you are being trained in biomedical research, you will have the opportunity to explore cutting edge techniques and gain knowledge of the latest instrumentation by utilizing the equipment in our labs and the core facilities that provide support to our entire NCV faculty.

You will gain experience by learning how to conduct research and from many additional rewarding, enjoyable opportunities for our students including social and recreational activities, and cultural events, as well as self-help seminars that will prepare you in all aspects of graduate school life and beyond including resume writing, graduate school applications, test taking, grant writing and more.

Benefits

  • Competitive stipend: $4,700
  • Suite-style room and meal plan
  • Travel expenses to and from Lincoln
  • Campus parking and/or bus pass
  • Full access to the Campus Recreation Center and campus library system
  • Wireless internet access

Learn more about academic and financial benefits.

Events

  • Department seminars and presentations
  • Professional development workshops (e.g., applying to graduate school, taking the GRE)
  • Welcome picnic
  • Day trip to Omaha's Henry Doorly Zoo
  • Canoe and camping trip
  • Research symposium

Mentors and Projects

Dr. Peter C. Angeletti School of Biological Sciences

HPV replication, encapsidation, and pathogeneses

Dr. Angeletti’s research is focused on topics relating to sexually transmitted Human papillomaviruses (HPVs). The first topic involves the analysis of cis and trans-acting signals required for stable replication of HPVs.  A second topic of interest is the analysis of the packaging requirements for HPVs.  A final area of interest for the lab is in discovery of the rates of genital HPV infection and genotypes present in HIV positive populations in Zambia, Africa.  In these studies we hope to determine if HIV plays a role in susceptibility to HPV infection and whether it influences progression of HPV lesions to cancer. 

Dr. Deborah Brown Nebraska Center for Virology

Development of influenza vaccines that promote cross protection and induce T cell memory responses to conserved antigens

Our research project is focused on the host immune response to viral infections.  Primarily, we use mouse models of influenza infection to understand how CD4 T cells are activated during infection and the mechanisms employed by these cells to help clear infectious virus.  We also use a T cell receptor (TCR) transgenic (Tg) mouse model in which all of the CD4 T cells recognize a piece of the influenza virus and various "knock-out" mouse strains lacking important immune response genes to determine which type of CD4 T cells provide protection to lethal influenza infections which will further our understanding of CD4 T cell biology and provide a framework for developing oval vaccine formulations to combat highly pathogenic and emerging influenza virus strains.

Dr. Hernan Garcia-Ruiz Plant Pathology

Plant-virus interactions, anti-viral RNA silencing, RNA virus replication and their interconnections

Hernan is a virologist interested in the molecular mechanisms of viral RNA replication and in antiviral RNA silencing. Hernan comes to UNL from the Donald Danforth Plant Science Center, where he was a research scientist. Hernan completed his postdoctoral work at the Oregon State University Center for Genomics and Biocomputing, with support from a Helen Hay Whitney fellowship. At UNL Hernan is the State Virologist and will teach a Molecular Virology class. Initial research will focus on the interconnection between RNA replication and RNA silencing mechanisms in viruses using yeast and plants as model systems in combination with genomic and bioinformatics approaches.

Dr. Qingsheng Li School of Biological Sciences

Interaction of HIV-1 with its host during mucosal transmission, and development of anti-viral topical microbicide and vaccine

Qingsheng’s research focuses on better understanding the interaction of human immunodeficiency virus type-1 (HIV-1) with its host in the earliest infection to elucidate key steps and critical events in the mucosal transmission of HIV-1, to identify correlates of protection, and ultimately to develop an effective anti-viral topical microbicide and vaccine.

Dr. Fernando Osorio School of Veterinary Medicine and Biomedical Sciences

Tropism, immunopathogenesis, and vaccinology of porcine reproductive and respiratory syndrome virus (PRRSV)

My research centers on pathogenesis of and immune response to viral infections. Due to the significance of the subject for U.S. animal agriculture, we focus on a major viral agent that affects swine: Porcine Reproductive and Respiratory Syndrome Virus (PRRSV, an arterivirus, ssRNA+ genome).

Dr. Asit Pattnaik School of Veterinary Medicine and Biomedical Sciences

Replication and pathogenic mechanisms of RNA viruses

Prerequisites: https://www.unl.edu/virologycenter/asit-k-pattnaik-phd

Studies in my laboratory focus on molecular biology and immunopathogenesis of two different viral pathogens, the vesicular stomatitis virus (VSV), a non-segmented negative-strand RNA virus in the family Rhabdoviridae and order Mononagavirales, and the porcine reproductive and respiratory syndrome virus (PRRSV), a positive-strand RNA virus in the family Arteriviridae and order Nidovirales. VSV has served as an excellent paradigm for many negative-strand RNA viruses (some of which are important human pathogens, such as respiratory syncytial, rabies, measles, and parainfluenza, hemorrhagic bunya and arenaviruses) to understand the basic mechanisms of the genetic expression of this group of viruses. PRRSV is an economically important pathogen, causing serious diseases in swine worldwide. Understanding the mechanism(s) of gene expression and its regulation as well as pathogenesis is essential for identifying virus-specific targets for therapeutic intervention in controlling infection by these viruses.

Dr. Thomas Petro Department of Oral Biology, College of Dentistry

Role of IRF-3 in pathogenesis, as well as innate and adaptive immunity during Theiler’s Virus infection

All humans are infected with viruses throughout their lifetime. While most of these virus infections stimulate innate and adaptive immunity and are eliminated, it is estimated that each human is infected by 8-10 viruses that persistently stimulate the immune systems and are never eliminated. It is hypothesized that in some cases these persistent viral infections lead to diseases, such as Multiple Sclerosis (MS). As a model for persistent viral infections, his laboratory is focusing upon the innate immune response to the Theiler's Murine Encephalomyelitis Virus (TMEV). In some strains of mice, TMEV persistently infects macrophages of the immune system but is never eliminated within the lifetime of the individual mouse. Those mice strains end up with demyelination and symptoms like MS. In other strains of mice, TMEV is eliminated within a few weeks. However, the immune response to eliminate the virus is not without consequence and these mice end up with damage to the hippocampus region of the brain. We showed that failures within the innate immune system are causes of TMEV persistence but also protect the mice from immune mediated damage to host tissues. 

Dr. N R Jayagopala Reddy School of Veterinary Medicine and Biomedical Sciences

Cellular and molecular mechanisms of auto-immunity

Prerequisites: https://jayreddy.unl.edu/

Most healthy individuals have a propensity to develop autoimmune diseases, as evidenced by the presence of autoreactive T cells in their naïve periphery. T cells are educated in the thymus, where high-affinity T cell receptor (TCR)-bearing cells that interact with self-antigens are deleted by negative selection, while T cells with low-affinity TCR to self-antigens are selected positively and exported to the periphery. In spite of the presence of self-reactive T cells in the naïve peripheral repertoires, autoimmune responses do not ensue spontaneously. How this tolerance is maintained is a fundamental question. It has been suggested that the environmental antigens that bear similarities to self-antigens break self-tolerance by cross-reactivity. To this end, we focus our investigations on the identification of microbial products that can potentially induce autoimmunity in the heart and immunologically privileged sites such as the central nervous system and eyes through antigenic mimicry. Mechanistically, we are particularly interested in delineating the role of antigen-specific T cells in the autoimmune diseases of infectious origin.

Dr. James Van Etten Plant Pathology

Characterization of large dsDNA viruses that infect certain chlorella-like green algae

Research in the Van Etten laboratory focuses on the isolation and characterization of large icosahedral, dsDNA-containing, plaque-forming viruses that infect certain unicellular, eukaryotic chlorella-like green algae.  These viruses are ubiquitous in fresh water from all over the world. The chlorella viruses have genomes as large as 370 kb that contain as many as 400 protein encoding- and 16 tRNA encoding-genes.  Besides their large genomes, the chlorella viruses have other unexpected features: (i) They encode multiple DNA methyltransferases and DNA restriction endonucleases.  (ii) Unlike other glycoprotein-containing viruses, chlorella viruses encode most, if not all, of the components required to glycosylate their proteins.  (iii) Many chlorella virus-encoded proteins are either the smallest or among the smallest proteins of their class.  Consequently, these proteins serve as models for mechanistic and structural studies.  Infection by the chlorella viruses resembles bacterial infection by tailed bacteriophages in many respects.

Dr. Hiep Vu Department of Animal Science

Immune response against porcine respiratory and reproductive syndrome virus (PRRSV), and development of vaccines

My laboratory studies two important viruses of swine: porcine reproductive and respiratory syndrome virus (PRRSV) and influenza A virus of swine (IAV-S). The research topics that are studied in my laboratory include: (i) Host immune responses to natural infection or vaccination, (ii) Molecular characteristics of the viruses currently circulating in the swine population, and (iii) Viral proteins and/or epitopes capable of eliciting protective immunity. Collectively, results obtained from these studies will be valuable for the optimal design safe and effective vaccines against divergent viral strains circulating in the field.

Dr. Eric Weaver School of Biological Sciences

Adenovirus vector for vaccine against influenza and other viruses

My current research explores the use of bioinformatics, phylogenetics, immunology and molecular biology to study the basic mechanisms of host-pathogen interactions and to create improved vaccines against microbial diseases. My research program can be broken into two arms, vaccine antigen design and vaccine platform design. First, we are testing ancestral/centralized genes to determine if they are useful as universal vaccines  in vivo.  In addition we are exploring systems biology approaches to improve vaccine antigen design to improve the breadth and efficacy of the vaccine antigens. Second, vaccine platform design explores the use of alternative viral vectors as vaccines, as well as the development of safer more effective viral vaccine platforms.

Dr. Matthew Wiebe School of Veterinary Medicine and Biomedical Sciences

Understanding intracellular defenses against foreign DNA by using poxvirus-infected cell models

One of the most fundamental questions in the field of immunology is how our immune system identifies an invading pathogen as “foreign” rather than “self”.  When this decision is made correctly growth of the virus or bacteria can kept at a minimum.  However, failure to recognize an invader extends the amount of damage it may produce or, if self is incorrectly regarded as foreign, then autoimmune disease occurs.  While significant progress has been made toward identifying viral and bacterial components which are found to be “foreign” and activate the immune system, much remains to be learned.

Dr. Charles Wood School of Biological Sciences

HIV and Kaposi’s sarcoma associated human herpesvirus transmission, evolution, and disease pathogenesis

The Wood laboratory focuses on HIV/AIDS and AIDS associated cancers in Africa. The lab studies the transmission and evolution of HIV, and the risk factors that are involved. Research involves collaboration with University Teaching Hospital in Zambia and the Ocean Road Cancer Institute in Tanzania, countries in Africa where HIV/AIDS and AIDS associated cancers such as Kaposi’s sarcoma (KS) are prevalent. They study neuroAIDS and whether the brain can serve as an HIV reservoir, how HIV is transmitted from mothers to their infants, and how the virus evolves into new strains to develop drug resistance in infected individuals in Zambia. A parallel project is to study the transmission of a human herpesvirus, known as the Kaposi’s sarcoma virus in both Zambia and Tanzania, which is linked to KS in AIDS patients. The focus is to determine how frequent is the infection by this virus, its route of transmission, the mechanism that this viruses causes cancer and how HIV acts as a co-factor, especially in AIDS patients.

Dr. Shi-hua Xiang School of Veterinary Medicine and Biomedical Sciences

HIV envelope glycoprotein structure, function, vaccine design, and utilizing commensal bacteria for anti-HIV infection

Human immunodeficiency virus type 1 (HIV-1) is the etiologic agent of AIDS (Acquired Immune Deficiency Syndrome).  About thirty years after identification of HIV as the causative agent of AIDS, the AIDS epidemic remains a global health issue.  Our research interests are focused on HIV/AIDS, with the ultimate goal of developing an effective vaccine or a long-term preventive strategy to counter this devastating pandemic.  In addition, we are also working on small ruminant lentviruses (SRLV) and try to find the genetic resistant-forms against SRLV infection.

Dr. Luwen Zhang School of Biological Sciences

Herpesvirus-host interactions in innate immunity and cancer formation

Research Interests:  Dr. Luwen Zhang’s laboratory studies the transformation processes. Epstein-Barr virus is (EBV) a human herpesvirus of increasing medical importance. EBV infection has been associated with the development of several human cancers. In immunocompromised individuals, such as organ transplant recipients or AIDS patients, EBV almost certainly causes two fatal cancers: post-transplantation lymphoproliferative disorder (PTLD) and AIDS-associated central nerve system (CNS) lymphoma.  The Zhang lab tackles the problems related to how virus interacts with cell, and transforms normal cells into cancerous ones.  Also, potential treatment of human cancers is also on their agenda. Zhang lab has been testing a novel approach to specifically block the viral transformation events that lead to the development of human cancers.