REU: Systems Biology of Plant and Microbiome

Center for Root and Rhizobiome Innovation  – https://crri.unl.edu/

For information contact

Nicole Busboom

Outreach Coordinator, Nebraska EPSCoR
402-472-8946

See Projects
Plant & Microbiome Students at REU picnic
Plant & Microbiome Students at REU picnic

Who should apply


Related fields

  • Plant Pathology
  • Biochemistry
  • Biology
  • Agronomy and Horticulture
  • Biology Systems Engineering
  • Chemistry
  • Genetics

Eligibility

Participation in the Nebraska Summer Research Program is limited to students who meet the following criteria:
  • U.S. Citizen or Permanent Resident
  • Current undergraduate with at least one semester of coursework remaining before obtaining a bachelor's degree

See Eligibility for more information.

How to apply

Follow the application steps to submit the following materials.

About the Program

The Center for Root and Rhizobiome Innovation (CRRI) will establish and develop tools and technologies for more rapid, precise, and predictable crop genetic improvement that complement methods currently used by biotechnologists and plant breeders.  These innovations are needed because of the urgency and enormity of challenges facing global agriculture, including the need to feed a rapidly growing population in the face of extreme climate variations and limitations in water and soil vitality.  

CRRI research will be structured around a systems and synthetic biology core to generate and iteratively improve network models of plant metabolism for predictable outcomes from genetic modifications.  CRRI’s systems and synthetic biology research will be applied to the study of root metabolism and its influence on root-interactions with soil microbes for improved plant health.    

Research will focus on root metabolism in maize, a plant genetic model and important crop species, but findings will be broadly applicable to other plants and crop species.  CRRI will develop and use fundamental knowledge to create translational products with far-reaching impact on plant and microbial biology and global agriculture.

Benefits

  • Competitive stipend: $5,000
  • Suite-style room and meal plan
  • Travel expenses to and from Lincoln
  • Campus parking and/or bus pass
  • Full access to the Campus Recreation Center and campus library system
  • Wireless internet access

Learn more about academic and financial benefits.

Events

  • Department seminars and presentations
  • Professional development workshops (e.g., applying to graduate school, taking the GRE)
  • Welcome picnic
  • Day trip to Omaha's Henry Doorly Zoo and Aquarium
  • Outdoor adventures
  • Research symposium

Mentors and Projects

Investigating the photoprotection characteristics under control and stress conditions

We seek to understand how we can modify photosynthesis by genome alteration or/and breeding for improving plant growth under abiotic stresses to secure food and bioenergy production in the future.

Particularly, we focus on the mechanism of photoprotection.  However, photoprotection protects plants from excessive light energy by its harmless dissipation as heat, it can also compete for energy with photosynthesis when the light is limited (e.g. overcast day). Since the speed of induction and relaxation of photoprotection can have a significant effect on plant growth, we are interested in defining desired characteristics of photoprotection under varied growth conditions.

The project will include measuring plants photoprotection based on chlorophyll fluorescence, analyzing images and investigating the biochemical changes in the leaves. It is expected that, over time, the student will master basic skills and be able to take on more responsibility and independence.

Dr. Marc Libault Agronomy and Horticulture

Developing and analyzing the transcriptome of each cell composing the maize root biology

This project is important to access because it will help to understand the role of each maize gene in regulating root development and root cell differentiation.

To reach his/her objective, the student will benefit from the expertise of lab members in plant single-cell –omics (one of the most innovative technologies currently existing in molecular analyses) and in bioinformatics. The students will not only perform experiments in the laboratory, but he/she will analyze his/her results to create a unique understanding of the role of maize genes during root development. His/her dataset will be integrated with other plant root transcriptomes to provide a deeper understanding of the evolution of plant genes. This project is relevant to on-going CRRI efforts because it will complement the transcriptomic analyses currently conducted as part of the CRRI project.

Dr. Rajib Saha Chemical and Biomolecular Engineering

Creating Genetic Circuit Designs Using Systems and Synthetic Biology Tools

The student will work to improve and apply a quantitative tool for the design of genetic circuits for Zea mays (known as corn or maize) plants the long-term goal of improvement of the root-rhizobiome system for improved plant health, yield, decreased fertilizer requirements, and the testing of other hypotheses which may arise. Over the REU term, the student will be familiarized with a wide variety of topics in systems and synthetic biology fields including optimization, set theory, genetic regulation, binary logic, genetic circuits, and metabolic modeling. Students will create genetic circuit designs using systems and synthetic biology tools and will be informed by models of root-rhizobiome metabolism. Their designs will be used to create a library of genetic circuit designs, including their components, associated logic flow diagrams, response patterns, and recommendations for their use.

Dr. Bin Yu Biological Sciences

The Molecular Mechanisms of RNA Metabolism and Functions

Student would work to understand the molecular mechanisms underlying small RNA metabolism and function. RNA silencing is a process triggered by ~21-24 nucleotide RNAs to repress gene expression. The Yu lab is interested in understanding of the mechanisms governing RNA silencing and development of RNA silencing based-technologies that can be used to improve crop traits.

Dr. Chi Zhang Department of Biological Sciences

Developing bioinformatics pipelines to the analysis of RNA-seq datasets to improve gene structure annotation in plants

Precursor messenger RNA (pre-mRNA) splicing is the process by which intron sequences are identified and excised from pre-mRNA transcripts with concurrent ligation of the flanking exons. Pre-mRNA is an important step for gene expression regulation, and gene structure information, such as exon sequences and locations, is critical in pre-mRNA splicing studies. Next-generation sequencing technology has been used in biological studies widely, including obtaining gene structure information. However, many exons, such as exon with structure variations, are challenging to be detected with current methods. Bioinformatics tools are employed to characterize gene structure information in a high-throughput fashion. It is urgent to get better bioinformatics tools to analyze next generation sequencing data to get better characterize gene structures.